Effect of Fusarium species isolated from switchgrass plants and seeds on foliar disease and stand establishment

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B. Ownley, S. Collins, and M. Dee, “Effect of Fusarium species isolated from switchgrass plants and seeds on foliar disease and stand establishment,” Phytopathology, vol. 104, no. 11S, pp. S3.1–S3.138, Oct. 2014.

Type Journal Article
Author B. Ownley
Author S. Collins
Author M. Dee
URL http://dx.doi.org/10.1094/PHYTO-104-11-S3.1
Volume 104
Issue 11S
Pages S3.1-S3.138
Publication Phytopathology
ISSN 0031-949X
Date October 22, 2014
Journal Abbr Phytopathology
DOI 10.1094/PHYTO-104-11-S3.1
Accessed 9/27/2015, 8:00:00 PM
Abstract Fusarium spp. have been reported from switchgrass plants and seeds, but information on their potential effects on stand establishment is lacking. Studies are needed to distinguish between fungi isolated from switchgrass that have minimal impact on stand establishment and biomass yield, from those that negatively impact the health of seeds, seedlings and mature plants. The aim of this study was to determine the pathogenicity and virulence of Fusarium spp., isolated from lesions of switchgrass field plants or commercial seed. Thirty isolates of Fusarium spp. were evaluated for pathogenicity and virulence on switchgrass with cut leaf assays. For each isolate, five 10-µl droplets with 104 spores/ml were applied to replicate cut leaf sections, maintained in a moist chamber for 5 days, and percent lesion area was determined with Assess software. Isolates of F. graminearum were the most virulent with lesion area of 38 to 42%. Virulent isolates were evaluated further with a seed disease assay. Two rates of inoculum, 101 spores/ml and 105 spores/ml, were applied to 100 seeds and incubated for 24 h. Twenty inoculated seeds were planted in soil mix in five replicate pots. Plant stand was significantly reduced by most isolates at the high inoculum rate; while F. equiseti and F. acuminatum isolates did reduce stand significantly, F. graminearum isolates resulted in <10% plant stand. These results suggest that Fusarium spp. play a role in poor stand establishment of switchgrass.


KEYWORDS:

 

  • Fungus
  • Fusarium
  • Panicum virgatum
  • Seed
  • Spore
  • Virulence

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